Keep the following in mind as you build your dichotomous keys. Campylobacter fetus Cyanobactena Planktotñrix agardñii go to 6.
Go to 7 Clostridium tetani Clostridium pemringens Filaments are straight curved or regularly spiraled.
Dichotomous key for bacteria. Dichotomous Key Simple Stain Cocci Bacilli Gram Stain Gram negative cocci Gram positive cocci Mannitol Salt yellow pink Staphylococcus aureus Staphylococcus epidermis Gram Stain Gram negative bacilli Gram positive bacilli 0DFRQNHV No color change Salmonella pullorum Pink colonies E. Coli Enterobacter aerogenes Acid Fast stain Acid Fast Mycobacterium tuberculosis. In this dichotomous example Begin the process of identifying unknown bacteria by observing their physical characteristics such as cell wall shape and linkages.
Use standard laboratory procedures like cell staining culturing and DNA sequencing to further narrow down your identification. Bacteria are generally grouped into species. This video shows you how to construct of bacteria dichotomous key using biochemical test results.
Gram Dichotomous Keys. Gram-negative rod Lactose oxidase- Lactose - oxidase nitrate nitrate- Indole Escherichia coli Indole- Klebsiella pneumoniae Urease- H2S- Shigella dysenteriae motility- citrate- Glucose Pseudomonas Alcaligenes aeruginosa faecalis Other tests for oxidase positives. Bacteria Dichotomous Key goto 2 go to 5 Escherichia coli.
Go to 3 go to. Campylobacter fetus Cyanobactena Planktotñrix agardñii go to 6. Streptococcus pneumomae Bacillus anthracis.
Go to 7 Clostridium tetani Clostridium pemringens Filaments are straight curved or regularly spiraled. Filaments are straight and solitary Form spores. How To Make A Dichotomous Key For Bacteria Easy Tutorial How To Make A Dichotomous Key For Bacteria.
Students and professionals use the dichotomous key to identify and classify objects ie. People animals plants bacteria etc into specific categories based on their characteristics. Its the most commonly used form of classification or type of identification key used in biology as it simplifies identifying unknown organisms.
BIO 275 Name_____ Making a Dichotomous Key —Bacteria Now that youve learned about some of the main characteristics of bacterial classification you will need to apply what you know. Your job is to make a dichotomous key to identify the 12 illustrated bacteria on the last page. Your first job is to outline the terms used to classify bacteria so you can use them in your.
Lab 2 Download Lab 2. First Unknown Dichotomous Keys for labs 2-4 Printable Version. These links will take you to a printable form of the dichotomous keys.
Making a Dichotomous Key — Bacteria Now that youve learned about some of the main characteristics of bacterial classification you will need to apply what you know. Your job is to make a dichotomous key to identify the 12 illustrated bacteria on the last page. Dichotomous keys are very useful because they allow non-expert users to identify organisms by directing them to look at the known important organisms.
Many people might not know how to distinguish different species of pine trees based on the arrangement of needles on a branch for example but the key would be able to ask them that question and give them useful information based. The dichotomous key is a chart or classification table that shows the category-wise classification of different objects. Scientists and specialists can classify animals plants viruses and other species using these dichotomous key charts.
They can develop the organisms classification based on their bone structure food habits habitats and more. Dichotomous Key to Identifying Eight Bacteria Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas fluorescens Corynebacterium xerosis Streptococcus pneumoniae Aeromonas hydrophila Gram stain negative positive Alcaligenes faecalis Mycobacterium smegmatis Citrobacter freundii Mycobacterium leprae Pseudomonas. Using the Mock Dichotomous Key in this Guide you will see that you should begin your own key by writing out the names of all the possible bacteria in the test near the top of the page.
I recommend using landscape sideways mode in laying out a key since the wider the page the better. When constructing a dichotomous key that may be used to identify bacteria we start by dividing a group of bacteria into two groups based on a certain characteristic such as the Gram-stain reaction. Then each of the two subgroups is subdivided into two groups based on a second characteristic and so on.
An initial identification key was defined using arginine dihydrolase lysine decarboxylase and ornithine decarboxylase tests as well as defining eight different clusters. This key leads each cluster to a secondary key for species identification. Most of Vibrio spp.
Presented an identification threshold of. Bacteria enter root cells. Enlarged root cells.
Bacteria change into bacteroids. Packed root cells enlarge. Rhizobia attach to root hair.
Pea plant Root hairs Rhizobia. Infection thread Bacteroids Root. Bacteria dichotomous key 1a.
If the bacteria is round go to 2. If the bacteria is not round go to 5. If the bacteria is paired in twos go to 3.
If the bacteria is not paired go to 4. If the bacteria is without a heavy covering it is diplococcus meningitidis and causes meningitis. A dichotomous key is used to identify and classify objects ie.
People animals plants bacteria etc into specific categories based on their characteristics. Its the most commonly used form of classification or type of identification key used in biology as it simplifies identifying unknown organisms. 11-1Differentiate the alphaproteobacteria described in this chapter by drawing a dichotomous key.
11-2Differentiate the betaproteobacteria described in this chapter by drawing a dichotomous key. 11-3Differentiate the gammaproteobacteria described in this chapter by drawing a dichotomous key. 11-4Differentiate the deltaproteobacteria described.
In order to identify unknown organisms from the list of twelve bacteria you will need to develop three dichotomous keys flowcharts one for each of the following groups of bacteria. Keep the following in mind as you build your dichotomous keys.